磁気共鳴分光特集：総説

In vivo NMR

In vivo NMR

菊地 　淳・赤嶺 健二
Jun KIKUCHI, and Kenji AKAMINE

生物学研究にとって、NMR法の非侵襲計測は魅力的な特性である。本総説では、(1) 高分解能NMR、(2) マジック角回転（MAS）法、(3) 磁気共鳴イメージング法における in vivo 計測の既報例を広く紹介し、我々のイネ種子を用いた初歩的試みについても述べた。さらに、NMRのソフト・ハード両面の進展と、これらが今後の in vivo 計測にもたらす展望についても議論した。

Non-invasive measurement is one of specific character of Nuclear Magnetic Resonance (NMR) especially in biological studies. Here, we review a variety of reported articles for (1) high-resolution solution NMR, (2) high-resolution Magic Angle Spinning (hr-MAS) and (3) Magnetic Resonance Imaging (MRI) in the intact biological systems. Furthermore, our preliminary investigations of these experiments for both non-labeled and uniformly 15N-labeled rice seeds are described. In addition to this, future perspectives of methodological advances of both NMR software and NMR hardware are discussed.

Keywords:Stable isotope labeling, Magic angle spinning, Magnetic resonance imaging, Metabolism, Plant seed